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Description / Abstract
The human papillomavirus is a leading cause of cervical cancer around the world. This oncovirus uses proteins like E7 to hijack the cell cycle by binding to cellular proteins, such as pRb. Another potential binding target of E7 is CXCL14. This chemokine plays a key role in the viral immune response which would be a significant target for E7. The purification of this protein for binding studies was developed through fast protein liquid chromatography (FPLC), using a nickel column for affinity binding and a size exclusion column, and the protein sample was then visualized by NMR. The protocol was successful in purifying CXCL14 which can be used for further study in the binding of E7 to CXCL14.
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Download as a PDF: Honors Paper
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KeywordsHPV, CXCL14, E7 protein binding
Degree AwardedB.S. in Biology-Chemistry
Awarding InstitutionPoint Loma Nazarene University
Committee MembersAriane Jansma, Heidi Woelbern, Kristopher Koudelka
Periodical TitleArete: The PLNU Honors Journal
Peer Reviewed?No
Collections With This Resource
PLNU Undergraduate Honors Projects 2021-2025
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