- Resource Types
- Resource Languages
- Institutional Repository
Keep Browsing
WHDL - 00017638
Visit the home page
About Site Language
WHDL is viewable in multiple languages. Use the pull-down menu to select a language to view the site.
I changed my language, but I’m still seeing resources in the other languages?
If a resource or text has not been translated into your selected language, it will appear in the initially added language. We are always looking for help translating these resources. If you can help, contact us!
Cover Image
Description / Abstract
The ELOVL4 enzyme is required to generate very long-chain fatty acids with structural and functional importance in the retina. Genetic defects in ELOVL4 are responsible for Stargardt’s disease, leading to a loss of central vision. This paper focuses on creating an in-vitro model of Stargardt's disease that replicates a key genetic defect observed in patients to test dietary supplements or other treatments that can restore normal long-chain fatty acids in the retina. We are using CRISPR to create a patient-relevant 5 base pair deletion on ELOVL4 in induced pluripotent stem cells (IPSCs) that can be grown into retinal organoids in culture. Bioinformatics tools were utilized to generate potential CRISPR guides and repair oligonucleotides that are predicted to effectively cut and repair the gene with the 5bp mutation observed in patients.
Download this Resource
Download Free from WHDL
-
Download as a PDF
File Size Small - 1.7 MB
- Publish Year
- Subject Index
KeywordsCRISPR Cas9, Stargardt's disease, ELOVL4 gene, macular dystrophy, Stargardt's, Stargardt, CRISPR, CRISPR/Cas9, Retina, Organoid, iPSCs
Degree AwardedB.S. in Biology
Awarding InstitutionPoint Loma Nazarene University
Committee MembersMike Dorrell, Dawne Page, Heidi Woelbern
Peer Reviewed?No
Collections With This Resource
PLNU Undergraduate Honors Projects 2021-2025
69 Resources
- English
Related Resources
Dairy Farming: An Assessment of the Sustainability of Dairy Farms Across the United States and in Bond County
- Academic (Thesis, Dissertation, Etc.)
- Unpublished Text
- English
2020
The Effects of Kavalactones on Cholinergic Signaling and Aceylcholinesterase Activity in Caenorhabditis elegans
- Academic (Thesis, Dissertation, Etc.)
- Unpublished Text
- English
2020
The Endangered Species Act and Its Effect on Birds in the United States
- Academic (Thesis, Dissertation, Etc.)
- English
Predator-prey relationships for coyote (Canis latrans), ocelot (Leopardus pardalis), and puma (Puma concolor) in Costa Rican ecosystems
- Academic (Thesis, Dissertation, Etc.)
- English
A comparative study between two approaches to university STEM education in the Democratic Republic of Congo: The effects of inquiry-learning on student learning outcomes and plagiarism rates
- Academic (Thesis, Dissertation, Etc.)
- English
2022
A Revision of the Conceptual Inventory of Natural Selection for Middle School Students
- Academic (Thesis, Dissertation, Etc.)
- English
A Study of Genes Expressed in Immunoglobulin M B Cells
- Academic (Thesis, Dissertation, Etc.)
- English
A Study of Pencil Urchin Population Genetics at Hannibal Bank
- Academic (Thesis, Dissertation, Etc.)
- English
A Study of the Diversity and Community Structure of Fauna at Hannibal Bank Based on Imagery Data
- Academic (Thesis, Dissertation, Etc.)
- English
Addressing Quantitative Literacy Gaps: Use of an Intervention to meet the needs of Struggling STEM Majors
- Academic (Thesis, Dissertation, Etc.)
- English
2024
An Anatomical and Physiological Study on the Effects of Whiplash
- Academic (Thesis, Dissertation, Etc.)
- English
An Exploration of Graphing Software Program Selection and Relative Proficiency Among Undergraduate Biology Students
- Academic (Thesis, Dissertation, Etc.)
- English
Analysis of Plasmids Expressing Bacteriophage Lambda Procapsid
- Academic (Thesis, Dissertation, Etc.)
- English
2023
Behavior Enrichments for the Stress of Wolves
- Media
- English
Bioassay-guided Isolation of a Natural Product Inhibitor of Epicoccum Nigrum to Combat Citrus Greening Disease
- Academic (Thesis, Dissertation, Etc.)
- English
Cancer Proliferation Mediated by Tumor-Associated Macrophages (TAMs)
- Academic (Thesis, Dissertation, Etc.)
- English
Cloning gpB and gpC for the In Vitro Assembly of Bacteriophage Lambda Procapsids for use as Viral Nanoparticles
- Academic (Thesis, Dissertation, Etc.)
- English
2024
Comparison of Massed Versus Distributed Use of Natural Selection Concept Cartoons in a Secondary Biology Curriculum
- Academic (Thesis, Dissertation, Etc.)
- English
Comprehensive Single-Season Occupancy Analysis with Covariates for all Species within Genus Tapirus
- Academic (Thesis, Dissertation, Etc.)
- English
2023
Creationist-Believing Students’ Scientific Knowledge and Reasoning about Evolution
- Academic (Thesis, Dissertation, Etc.)
- English
Degradation of pRb by Retrovirally Infected Cell Lines
- Academic (Thesis, Dissertation, Etc.)
- English
Development and field testing of a concept cartoon intervention to improve students’ understandings of the role of genetics in natural selection as a mechanism of evolution
- Academic (Thesis, Dissertation, Etc.)
- English
2021
Elucidating Population Genetics of Shrimp Species On Underwater Volcanoes
- Academic (Thesis, Dissertation, Etc.)
- English
2024
Exploring Transcriptional Regulators that Control Emergent Behaviors in Myxococcus xanthus
- Academic (Thesis, Dissertation, Etc.)
- English
Expression of PDZ domain 1 of the Scribble Polarity Complex
- Academic (Thesis, Dissertation, Etc.)
- English